Tehran University of Medical Sciences Journal of Biostatistics and Epidemiology 2383-4196 5 4 2020 07 22 304 309 Amineh Kousehlou Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Mohammad Gholami-Fesharaki Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2020 02 24 2020 06 07 Introduction:A very limited evidences are available that considered the relationship between Shift Work (SW) and lipid variables. Objectives: As the importance of this subject, this prospectively cohort study examines the association between SW and Body Mass Index (BMI) and lipid profiles among male workers using multilevel ordinal model Methods: This five years prospective cohort study has been conducted in random selected workers (using cluster sampling) who work in Esfahan’s Mobarakeh Steel Company (EMSC) (Iran) between 2011 and 2015. Results: The study sample included 1626 male workers (mean age=41.5). Among these subjects, 652 (40.01%), 183 (11.3%) and 791 (48.6%) were day workers, weekly rotating shift workers and routinely rotating.  After controlling for several confounding variables, except HDL and Cholesterol, the odds ratio for high HDL was decreased by 26% (OR=0.74, P<0.001) and increased for TG by 36% in weekly rotating shift workers compared to day workers.   Conclusion: Since weekly rotating shift workers had higher mean value of TG in their serum compared to day workers, they should limit eating high-fat diets in order to decrease risk of CVD. https://jbe.tums.ac.ir/index.php/jbe/article/view/329 https://jbe.tums.ac.ir/index.php/jbe/article/download/329/245